tgfβ  (R&D Systems)

Journal: PLOS Neglected Tropical Diseases

Article Title: An integrated analysis of the structural changes and gene expression of spleen in human visceral leishmaniasis with and without HIV coinfection

doi: 10.1371/journal.pntd.0011877

Figure Lengend Snippet: Cell distribution in spleen compartments.

Article Snippet: Cytokine detection was performed using these primary antibodies: TGFβ (ab66043), IL1β (AF-201-NA), IFNγ (MAB 285), TNFα (AF-210-NA), IL10 (AF-217-NA), IL6 (AF-206-NA), IL17 (AF-317-NA) and IL4 (AF-204-NA) (RD Systems), as well as 14-4776-82 (Foxp3 from E-Bioscience) and Caspase 3, in accordance with the following protocol: Antigen unmasking was performed using a heat-induced antigen retrieval method in a water bath with Retrieval Buffer (Dako Corporation, Carpinteria, CA, USA) for 25 minutes at 95°C, pH 9.0.

Techniques:

Journal: Frontiers in bioscience (Landmark edition)

Article Title: C3aR Antagonist Alleviates C3a Induced Tubular Profibrotic Phenotype Transition via Restoring PPARα/CPT-1α Mediated Mitochondrial Fatty Acid Oxidation in Renin-Dependent Hypertension.

doi: 10.31083/j.fbl2810238

Figure Lengend Snippet: Fig. 2. The C3aR antagonist SB290157 mitigates the C3a analogue-induced profibrotic phenotype transition and Mito FAO inhibition in HK2 cells. (A) Representative western blotting images and summarized data of C3aR and TGFβ protein levels in HK2

Article Snippet: HK2 cells were treated with the C3a analogue (1 μg/mL, Sangon Biotech, Shanghai, China) or PBS for 24 h. For C3aR inhibition, cells were pretreated with the C3aR antagonist SB290157 (1 μM, Sigma‒Aldrich, St. Louis, MO, USA) for 1 h. HK2 cells were harvested for western blotting analysis, and the culture medium was used to evaluate the concentration of transforming growth factor β (TGFβ) using Human TGFβ ELISA kits (Proteintech, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Inhibition, Western Blot

Journal: Frontiers in bioscience (Landmark edition)

Article Title: C3aR Antagonist Alleviates C3a Induced Tubular Profibrotic Phenotype Transition via Restoring PPARα/CPT-1α Mediated Mitochondrial Fatty Acid Oxidation in Renin-Dependent Hypertension.

doi: 10.31083/j.fbl2810238

Figure Lengend Snippet: Fig. 5. C3aR and mesenchymal transition markers increase in the kidney cortex of 2K1C rats. (A) Representative western blotting images, summarized data of C3aR and α-SMA protein levels and (B) summarized C3aR, α-SMA, TGFβ and CD68 mRNA levels in the

Article Snippet: HK2 cells were treated with the C3a analogue (1 μg/mL, Sangon Biotech, Shanghai, China) or PBS for 24 h. For C3aR inhibition, cells were pretreated with the C3aR antagonist SB290157 (1 μM, Sigma‒Aldrich, St. Louis, MO, USA) for 1 h. HK2 cells were harvested for western blotting analysis, and the culture medium was used to evaluate the concentration of transforming growth factor β (TGFβ) using Human TGFβ ELISA kits (Proteintech, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Western Blot

Journal: Cell Death & Disease

Article Title: Early expansion of myeloid-derived suppressor cells inhibits SARS-CoV-2 specific T-cell response and may predict fatal COVID-19 outcome

doi: 10.1038/s41419-020-03125-1

Figure Lengend Snippet: A IFN-γ production from PBMC, PMN-MDSC depleted PBMC (DEPL), and DEPL + PMN-MDSC (1/4 ratio) from 11 patients upon stimulation with SARS-CoV-2 peptides or with PHA. Results are shown as Box and Whiskers. Kruskal–Walliswith Dunn’s post test was applied. B IFN-γ production from PBMC from 4 patients upon stimulation with SARS-CoV-2 peptides (CTR) and neutralizing anti-human TGF-β, Nor-NOHA, or L-NAME.

Article Snippet: Where indicated, PBMCs, PMN-MDSC depleted PBMCs, and Depleted PBMCs plus MDSC (ratio 1:4), were treated with N(ω)-hydroxy-nor-L-arginine (0.5 mM, cat. 399275, Calbiochem, Germany), neutralizing anti-human TGFβ antibody (10 μg/ml, cat. AB 101 NA, R&D Systems, USA), or L-N G -nitro arginine methyl ester (1 mM, cat.483125, Calbiochem, Germany).

Techniques:

Journal: Open Access Macedonian Journal of Medical Sciences

Article Title: The Expression of Transforming Growth Factor Beta-1 and Interleukin-6 on Human Prostate: Prostate Hyperplasia and Prostate Cancer

doi: 10.3889/oamjms.2019.548

Figure Lengend Snippet: Normality Test for Independent Variables

Article Snippet: Following primary antibody were used; Rabbit polyclonal anti-human TGFβ-1, Bioss, 0086R with a dilution of 1:200, and Rabbit polyclonal anti-human IL-6.

Techniques:

Journal: Open Access Macedonian Journal of Medical Sciences

Article Title: The Expression of Transforming Growth Factor Beta-1 and Interleukin-6 on Human Prostate: Prostate Hyperplasia and Prostate Cancer

doi: 10.3889/oamjms.2019.548

Figure Lengend Snippet: Post-Transformation Normality Test

Article Snippet: Following primary antibody were used; Rabbit polyclonal anti-human TGFβ-1, Bioss, 0086R with a dilution of 1:200, and Rabbit polyclonal anti-human IL-6.

Techniques:

Journal: Open Access Macedonian Journal of Medical Sciences

Article Title: The Expression of Transforming Growth Factor Beta-1 and Interleukin-6 on Human Prostate: Prostate Hyperplasia and Prostate Cancer

doi: 10.3889/oamjms.2019.548

Figure Lengend Snippet: The Expression of TGFβ-1 by Immunohistochemistry on Human Prostate Tissue. Prostate Hyperplasia (A) and Prostate Cancer (B). TGFβ-1 are mostly Expressed on Stromal Component Prostate Hyperplasia (A); The Stromal Staining is Greatly Reduced in Prostate Cancer, However Cancer Epithelial can Show TGFβ-1 Staining in Some Cases (B) Immunoperoxidase of Rabbit Anti Human TGFβ-1. Scale Bar 100 µm

Article Snippet: Following primary antibody were used; Rabbit polyclonal anti-human TGFβ-1, Bioss, 0086R with a dilution of 1:200, and Rabbit polyclonal anti-human IL-6.

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Open Access Macedonian Journal of Medical Sciences

Article Title: The Expression of Transforming Growth Factor Beta-1 and Interleukin-6 on Human Prostate: Prostate Hyperplasia and Prostate Cancer

doi: 10.3889/oamjms.2019.548

Figure Lengend Snippet: The Epithelial Expression of TGFβ-1 on Prostate Hyperplasia and Prostate Cancer Tissue

Article Snippet: Following primary antibody were used; Rabbit polyclonal anti-human TGFβ-1, Bioss, 0086R with a dilution of 1:200, and Rabbit polyclonal anti-human IL-6.

Techniques: Expressing

Journal: Open Access Macedonian Journal of Medical Sciences

Article Title: The Expression of Transforming Growth Factor Beta-1 and Interleukin-6 on Human Prostate: Prostate Hyperplasia and Prostate Cancer

doi: 10.3889/oamjms.2019.548

Figure Lengend Snippet: The Expression of TGFβ-1 by Immunohistochemistry on Human Prostate Tissue. Prostate Hyperplasia (A) and Prostate Cancer (B); The Stomal Area is Measured by Image J Software by Isolate the Brown Stained Area and Measuring the Proportion of Stained Area. There is a Higher Proportion of Stained Area in Prostate Hyperplasia (B) Compared to Prostate Cancer (D). Immunoperoxidase of Rabbit Anti Human TGFβ-1. Scale Bar 100 µm

Article Snippet: Following primary antibody were used; Rabbit polyclonal anti-human TGFβ-1, Bioss, 0086R with a dilution of 1:200, and Rabbit polyclonal anti-human IL-6.

Techniques: Expressing, Immunohistochemistry, Software, Staining

Journal: Open Access Macedonian Journal of Medical Sciences

Article Title: The Expression of Transforming Growth Factor Beta-1 and Interleukin-6 on Human Prostate: Prostate Hyperplasia and Prostate Cancer

doi: 10.3889/oamjms.2019.548

Figure Lengend Snippet: The Stromal Expression of TGF-β1 on Prostate Hyperplasia and Prostate Cancer Tissue

Article Snippet: Following primary antibody were used; Rabbit polyclonal anti-human TGFβ-1, Bioss, 0086R with a dilution of 1:200, and Rabbit polyclonal anti-human IL-6.

Techniques: Expressing